GENOMIC IN-SITU HYBRIDIZATION IN AVENA-SATIVA

被引：103
作者：
CHEN, QF [1 ]
ARMSTRONG, K [1 ]
机构：
[1] AGR CANADA, PLANT RES CTR, CENT EXPTL FARM, OTTAWA K1A 0C6, ON, CANADA
来源：
GENOME | 1994年 / 37卷 / 04期
关键词：
OATS; AVENA SATIVA L; IN SITU HYBRIDIZATION; FLUORESCEIN ISOTHIOCYANATE; GENOME ORGANIZATION;
D O I：
10.1139/g94-086
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Genomic fluorescent in situ hybridization was employed in the study of the genome organization and evolution of hexaploid oat (Avena sativa L. cv. Sun II, AACCDD, 2n = 6x = 42). Genomic DNAs from two diploid oat species, Avena strigosa (genomic constitution A(s)A(s), 2n = 14) and Avena pilosa (genomic constitution CpCp, 2n = 14), were used as probes in the study. The DNA from A. strigosa labelled 28 of the 42 (2/3) chromosomes of the hexaploid oat, while 14 of the 42 (1/3) chromosomes were labelled with A. pilosa DNA, indicating a close relationship between the A and D genomes. Results also suggested that at least 18 chromosomes (9 pairs) were involved in intergenomic interchanges between the A and C genomes.
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页码：607 / 612
页数：6
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