IMMUNODETECTION OF BIOTINYLATED LYMPHOCYTE-SURFACE PROTEINS BY ENHANCED CHEMILUMINESCENCE - A NONRADIOACTIVE METHOD FOR CELL-SURFACE PROTEIN-ANALYSIS

被引：100

作者：

MEIER, T ^{[1
]}

ARNI, S ^{[1
]}

MALARKANNAN, S ^{[1
]}

POINCELET, M ^{[1
]}

HOESSLI, D ^{[1
]}

机构：

[1] CTR MED UNIV GENEVA,DEPT PATHOL,CH-1211 GENEVA 4,SWITZERLAND

来源：

ANALYTICAL BIOCHEMISTRY | 1992年 / 204卷 / 01期

关键词：

D O I：

10.1016/0003-2697(92)90165-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Biotinylation and radioiodination have been compared for labeling lymphocyte-surface proteins and the labeled proteins symmetrically immunoprecipitated with antibodies recognizing major lymphocyte markers such as the murine Thy-1, CD25 (the α subunit of the interleukin-2 receptor), CD45, and human CD2 glycoproteins. The detection of biotinylated proteins by enhanced chemiluminescence after transfer to nitrocellulose was found to be fast and as efficient as the detection of iodinated proteins by autoradiography. The vectoriality of cell-surface biotinylation was ascertained by two-dimensional electrophoresis of the cellular extract in which the major cytoplasmic proteins were not found biotinylated. This nonradioactive labeling procedure offers a convenient and efficient alternative to radiola-beling of cell surfaces for the biochemical analysis of extracellular domains of membrane proteins. © 1992.

引用

页码：220 / 226

页数：7

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