EFFICIENT PRODUCTION OF SOMATIC HYBRIDS BETWEEN RAPHANUS-SATIVUS AND BRASSICA-OLERACEA

被引：0

作者：

YAMANAKA, H ^{[1
]}

KUGINUKI, Y ^{[1
]}

KANNO, T ^{[1
]}

NISHIO, T ^{[1
]}

机构：

[1] NATL RES INST VEGETABLES,ANO,MIE 51423,JAPAN

来源：

JAPANESE JOURNAL OF BREEDING | 1992年 / 42卷 / 02期

关键词：

BRASSICA-OLERACEA; RAPHANUS-SATIVUS; CELL FUSION; SOMATIC HYBRID; POLYETHYLENE GLYCOL; IODEACETAMIDE;

D O I：

暂无

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

An efficient procedure of somatic hybridizaiton between radish (Raphanus sativus) and cauliflower (Brassica oleracea) was developed by assessing the techniques at the steps from protoplast isolation to plant regeneration. Hypocotyl protoplasts of radish and cotyledon protoplasts of cauliflower were fused by treatment with polyethylene glycol solution containing dimethylsulfoxide. Cauliflower protoplasts having high plant regeneration ability were inactivated by iodoacetamide before fusion treatment. In eight fusion trials, 2965 calli were obtained, and after transfer to a shoot regeneration medium, 163 calli formed shoots. Of the 111 plants obtained, 62 had leaves of intermediate type between the two parents. The other 49 plants had similar leaves to cauliflower. Analysis of rDNA and acid phosphatase isozyme demonstrated the hybrid nature of the plants having leaves of intermediate type. Some of somatic hybrids had high pollen fertility and set seeds, indicating the possibility of utilizing these plants for breeding materials.

引用

页码：329 / 339

页数：11