PRESENCE OF LUTEINIZING-HORMONE-RELEASING HORMONE (LHRH) MESSENGER-RNA IN RAT SPLEEN LYMPHOCYTES

被引:77
作者
AZAD, N
EMANUELE, NV
HALLORAN, MM
TENTLER, J
KELLEY, MR
机构
[1] VET ADM MED CTR,MED SERV,HINES,IL 60141
[2] LOYOLA UNIV,STRITCH SCH MED,DEPT MED,MAYWOOD,IL 60153
[3] LOYOLA UNIV,STRITCH SCH MED,MOLEC BIOL PROGRAM,MAYWOOD,IL 60153
[4] LOYOLA UNIV,STRITCH SCH MED,DEPT MOLEC & CELLULAR BIOCHEM,MAYWOOD,IL 60153
关键词
D O I
10.1210/endo-128-3-1679
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Pursuant to our report of an immunoreactive and bioactive luteinizing hormone-releasing hormone (LHRH)-like molecule in rat spleen lymphocytes, we sought to determine whether these cells were capable of synthesizing LHRH by determining whether lymphocytes contain LHRH mRNA. To do this, total RNA was extracted from hypothalamic tissue, anterior pituitaries and from lymphocytes, and then this was reverse transcribed to cDNA and amplified via the polymerase chain reaction (PCR) utilizing synthetic oligonucleotides bracketing a portion of the LHRH gene. Following gel electrophoresis a discrete band of the expected size of 375 base pairs was found in the hypothalamus (positive control), and in lymphocytes, but not in the anterior pituitary (negative control). Furthermore, after Southern blotting, a P-32-labelled LHRH cDNA, hybridized to the 375 base pair, was amplified in hypothalamus fragments and in lymphocytes, but not in anterior pituitary tissue.
引用
收藏
页码:1679 / 1681
页数:3
相关论文
共 18 条
[1]   ISOLATION OF THE GENE AND HYPOTHALAMIC CDNA FOR THE COMMON PRECURSOR OF GONADOTROPIN-RELEASING-HORMONE AND PROLACTIN RELEASE-INHIBITING FACTOR IN HUMAN AND RAT [J].
ADELMAN, JP ;
MASON, AJ ;
HAYFLICK, JS ;
SEEBURG, PH .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (01) :179-183
[2]  
AZAD N, 1990, 72ND ANN M END SOC A, P361
[3]  
AZAD N, 1990, 2ND ANN M INT C NEUR, P60
[4]   THE RAT GONADOTROPIN-RELEASING HORMONE - SH LOCUS - STRUCTURE AND HYPOTHALAMIC EXPRESSION [J].
BOND, CT ;
HAYFLICK, JS ;
SEEBURG, PH ;
ADELMAN, JP .
MOLECULAR ENDOCRINOLOGY, 1989, 3 (08) :1257-1262
[5]   ORCHIDECTOMY AND IMMUNE-RESPONSE .1. EFFECT OF ORCHIDECTOMY ON LYMPHOID-TISSUES OF MICE [J].
CASTRO, JE .
PROCEEDINGS OF THE ROYAL SOCIETY SERIES B-BIOLOGICAL SCIENCES, 1974, 185 (1081) :425-+
[6]  
CHIODI H, 1976, FED PROC, V35, pA277
[7]   SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION [J].
CHOMCZYNSKI, P ;
SACCHI, N .
ANALYTICAL BIOCHEMISTRY, 1987, 162 (01) :156-159
[8]  
COSTA O, 1990, PROGR NEUROENDOCRINI, V3, P55
[9]   RAT SPLEEN LYMPHOCYTES CONTAIN AN IMMUNOACTIVE AND BIOACTIVE LUTEINIZING-HORMONE-RELEASING HORMONE [J].
EMANUELE, NV ;
EMANUELE, MA ;
TENTLER, J ;
KIRSTEINS, L ;
AZAD, N ;
LAWRENCE, AM .
ENDOCRINOLOGY, 1990, 126 (05) :2482-2486
[10]   REGULATION OF THE IMMUNE-SYSTEM BY SEX STEROIDS [J].
GROSSMAN, CJ .
ENDOCRINE REVIEWS, 1984, 5 (03) :435-455