EFFECT OF INTERLEUKIN-1-BETA-ON THE PRODUCTION OF CATHEPSIN-B BY RABBIT ARTICULAR CHONDROCYTES

被引：28

作者：

BAICI, A

LANG, A

机构：

[1] Department of Rheumatology, University Hospital

来源：

FEBS LETTERS | 1990年 / 277卷 / 1-2期

关键词：

CATHEPSIN-B; COLLAGENASE; ARTICULAR CHONDROCYTE; INTERLEUKIN-1-BETA; CELL CULTURE; RABBIT;

D O I：

10.1016/0014-5793(90)80816-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Rabbit articular chondrocytes in monolayer culture were stimulated with human recombinant interleukin-1-beta. Under the influence of the cytokine the intracellular pool of the cysteine endopeptidase cathepsin B was increased by a 2-4-fold factor, while enzyme secretion was not stimulated at a significant level. Under the same conditions, the secretion of collagenase, measured as an internal control, was stimulated about 6-fold. The effects of interleukin-1-beta-were compared to those caused by phenotypic modulation. Chondrocytes modulated by serial subcultures in monolayer secreted more cathepsin B, but less collagenase than differentiated cells (cultured within collagen gels). Thus, interleukin-1-beta-and phenotypic modulation affected differently two endopeptidases which are relevant in the pathogenesis of osteoarthritis.

引用

页码：93 / 96

页数：4

共 34 条

[31] STIMULATION OF PROCOLLAGENASE SYNTHESIS PARALLELS INCREASES IN CELLULAR PROCOLLAGENASE MESSENGER-RNA IN HUMAN ARTICULAR CHONDROCYTES EXPOSED TO RECOMBINANT INTERLEUKIN 1-BETA OR PHORBOL ESTER [J].