THE RELATIONSHIP BETWEEN THE HEMAGGLUTINATION-INHIBITION TEST AND THE ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR THE DETECTION OF ANTIBODY TO NEWCASTLE-DISEASE

被引：39

作者：

BROWN, J ^{[1
]}

RESURRECCION, RS ^{[1
]}

DICKSON, TG ^{[1
]}

机构：

[1] GEORGIA POULTRY LABS,OAKWOOD,GA 30566

来源：

AVIAN DISEASES | 1990年 / 34卷 / 03期

关键词：

D O I：

10.2307/1591248

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

An assay of 364 chicken serum samples for Newcastle disease virus antibodies determined that a commercial NDV enzyme-linked immunosorbent assay (ELISA) had a 98.2% sensitivity and a 91.7% specificity relative to the NDV HI test. The ELISA values regressed significantly (F = 930, df = 1/362, P less than 0.001) on the hemagglutination-inhibition (HI) titers. The correlational coefficient was 0.85. For individuals, two tests can have the same result based upon chance alone. Kappa is a measure of agreement between two tests that corrects for this chance agreement. The kappa between the ELISA and HI test was calculated to be 0.84 (Z = 7.74, P = 0.00001), which indicates a highly significant agreement between the two tests.

引用

页码：585 / 587

页数：3

共 7 条

[1] QUANTITATIVE ESTIMATION OF NEWCASTLE-DISEASE VIRUS-ANTIBODY LEVELS IN CHICKENS AND TURKEYS BY ELISA [J].