DETERMINATION OF LANSOPRAZOLE AND 5 METABOLITES IN PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：51

作者：

KAROL, MD

GRANNEMAN, GR

ALEXANDER, K

机构：

[1] Drug Metabolism Department, Abbott Laboratories, Abbott Park, IL 60064-3500, D4PK

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1995年 / 668卷 / 01期

关键词：

D O I：

10.1016/0378-4347(95)00068-T

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A high-performance liquid chromatographic method for the determination of lansoprazole, a new proton-pump inhibitor, and five of its metabolites in human plasma is described. Lansoprazole. its metabolites, and internal standard (omeprazole) were extracted into diethyl ether-methylene chloride and separation was obtained using a reversed-phase column under isocratic conditions. The method features monochromatic ultraviolet detection at 285 nm, and single extraction, single evaporation sample handling. The lower limit of quantitation, based on standards with acceptable coefficients of variation, was 10 ng/ml for all compounds. No endogenous compounds were found to interfere. This method has been demonstrated to be suitable for pharmacokinetic studies in humans.

引用

页码：182 / 186

页数：5

共 3 条

[1] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF LANSOPRAZOLE AND ITS METABOLITES IN HUMAN SERUM AND URINE [J].