ANALYSIS OF NEUTRAL SUGARS AS GLYCAMINES USING AN AMINO-ACID ANALYZER

被引：28

作者：

HARA, S

IKEGAMI, H

SHONO, A

MEGA, T

IKENAKA, T

MATSUSHIMA, Y

机构：

[1] Department of Chemistry, Osaka University College of Science, Toyonaka, Osaka

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 97卷 / 01期

关键词：

D O I：

10.1016/0003-2697(79)90341-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new method of the analysis of neutral sugars was developed based on the separation of their corresponding glycamines. Sugars and ammonia were combined by means of the reduction with sodlum cyanoborohydride. The glycamines thus obtained were quantitatively analyzed by an automatic amino acid analyzer. Satisfactery results were obtained in the analyses of the constituent sugars of several polysaccharides and glycoconjugates. © 1979.

引用

页码：166 / 172

页数：7

共 17 条

[1]

BAUES RJ, 1977, J BIOL CHEM, V252, P57

[2] CYANOHYDRIDOBORATE ANION AS A SELECTIVE REDUCING AGENT [J].