PLANTLET REGENERATION FROM SUSPENSION-CULTURES OF VALERIANA-WALLICHII DC

被引：9
作者：
MATHUR, J
机构：
[1] Plant Tissue Culture Laboratory, Department of Botany, University of Gorakhpur, Gorakhpur
来源：
PLANT SCIENCE | 1992年 / 81卷 / 01期
关键词：
MORPHOGENESIS; VALERIANA-WALLICHII; PLANT REGENERATION;
D O I：
10.1016/0168-9452(92)90030-P
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Suspension cultures of Valeriana wallichii DC. were initiated in liquid Murashige and Skoog's (MS) medium containing 16.1-mu-M alpha-naphthaleneacetic acid (NAA) and 0.93-mu-M kinetin. Cell aggregates showed rhizogenesis within 2-3 weeks giving rise to clumps of 4-6 roots. Isolated roots developed a green callus or a flattened leaf like structure at one end. Such bipolar structures when transferred to solid MS medium containing either 0.89-2.22-mu-M of 6-benzylaminopurine (BAP) or 0.93-4.60-mu-M of kinetin developed shoots and grew into complete plantlets.
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页码：111 / 115
页数：5
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