FUNCTIONAL-ANALYSIS OF THE YEAST GENOME - USE OF 2-DIMENSIONAL GEL-ELECTROPHORESIS TO DETECT GENES IN RANDOMLY CLONED DNA-SEQUENCES

被引：6

作者：

THORAVAL, D ^{[1
]}

REGNACQ, M ^{[1
]}

NEUVILLE, P ^{[1
]}

BOUCHERIE, H ^{[1
]}

机构：

[1] CNRS,GENET LAB,UA 542,AVE FAC,F-33405 TALENCE,FRANCE

来源：

CURRENT GENETICS | 1990年 / 18卷 / 04期

关键词：

2-D gel electrophoresis; Genome analysis; S; cerevisiae;

D O I：

10.1007/BF00318208

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Genes are overexpressed when present in yeast cells on multicopy plasmids. Taking advantage of the protein amplification which results from this overexpression, a method has been developed for large scale detection of yeast genes on randomly cloned DNA sequences. It is based on the analysis, by two-dimensional gel electrophoresis, of the proteins from yeast cells transformed with a yeast genomic DNA library constructed in a multicopy vector. We demonstrate here the applicability of this method for exploring the yeast genome. In addition, we report results which suggest that this method may also be useful for detecting regulatory genes. © 1990 Springer-Verlag.

引用

页码：281 / 286

页数：6

共 18 条

[1] TWO-DIMENSIONAL GEL ANALYSIS OF YEAST PROTEINS - APPLICATION TO THE STUDY OF CHANGES IN THE LEVELS OF MAJOR POLYPEPTIDES OF SACCHAROMYCES-CEREVISIAE DEPENDING ON THE FERMENTABLE OR NONFERMENTABLE NATURE OF THE CARBON SOURCE [J].