ANALYSIS AND CONFIGURATION ASSIGNMENTS OF THE AMINO-ACIDS IN A PYOVERDINE-TYPE SIDEROPHORE BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：10
作者：
HANCOCK, DK
REEDER, DJ
机构：
[1] National Institute of Standards and Technology (NIST), Biotechnology Division, Gaithersburg
来源：
JOURNAL OF CHROMATOGRAPHY | 1993年 / 646卷 / 02期
关键词：
D O I：
10.1016/0021-9673(93)83346-T
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The amino acid composition of the siderophore pyoverdine Pf244 was determined by reversed phase high-performance liquid chromatography (RP-HPLC) of phenylthiocarbamyl derivatives of the acid-hydrolyzed pyoverdine. The siderophore contains an acid-labile amino acid, N(delta)-hydroxyornithine. and an amino acid previously unknown in naturally occurring systems, L-threo-beta-hydroxyhistidine. Amino acid chirality assignments were determined by RP-HPLC of the beta-D-glucopyranosyl isothiocyanate tetraacetate derivatives. Reactions with amino acid oxidases established the L-configuration of threo-beta-hydroxyhistidine.
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页码：335 / 343
页数：9
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