5'-NUCLEOTIDASE IS INVOLVED IN CHICK-EMBRYO MYOBLAST SPREADING ON LAMININ

Previous studies have shown that 5′-nucleotidase, an ectoenzyme from chicken gizzard, interacts specifically with laminin and fibronectin, two glycoproteins of the extracellular matrix. Recently, we demonstrated that 5′-nucleotidase was involved in the spreading of chick embryo fibroblast on laminin. In the present communication, we report that a monoclonal antibody (CG37) raised-directed against 5′-nucleotidase inhibited the spreading of chick embryo myoblasts on laminin after their initial attachment to the substrate. Furthermore, monoclonal antibody CG37 specifically eluted 5′-nucleotidase from immobilized laminin and thus enabled its isolation from other myoblast laminin- binding proteins. © 1990.