A 3.5 GENOME EQUIVALENT MULTIACCESS YAC LIBRARY - CONSTRUCTION, CHARACTERIZATION, SCREENING AND STORAGE

被引：270
作者：
ANAND, R
RILEY, JH
BUTLER, R
SMITH, JC
MARKHAM, AF
机构：
[1] ICI Diagnostics, Northwich, Cheshire CW9 7RA, Gadbrook Park
来源：
NUCLEIC ACIDS RESEARCH | 1990年 / 18卷 / 08期
关键词：
D O I：
10.1093/nar/18.8.1951
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The construction of a yeast artifical chromosome (YAC) primary gridded library of 35,000 clones from human lymphoblastoid (48,XXXX) cell line DNA is described. The average YAC size is ∼350kb representing a >3.5 times coverage of the genme. The library is stored at -70°C as gridded clones on nylon filters impregnated with 20% glycerol and as glycerol suspensions of individual clones in microtitre plates providing a prolonged multi-user potential. To date we have used 14 single copy probes to screen this library by colony hybridisation as well as PCR and have isolated between 1 and 5 YAC clones for every probe. © 1990 Oxford University Press.
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页码：1951 / 1956
页数：6
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