IMMOBILIZATION OF TYROSINASE FOR USE IN NONAQUEOUS MEDIA - ENZYME DEACTIVATION PHENOMENA

被引：11

作者：

DAY, SH

LEGGE, RL

机构：

[1] Biochemical Engineering Group, Department of Chemical Engineering, University of Waterloo, Waterloo, N2L 3G1, Ontario

来源：

BIOTECHNOLOGY TECHNIQUES | 1995年 / 9卷 / 07期

关键词：

D O I：

10.1007/BF00159560

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have investigated features for minimizing the inactivation of tyrosinase (E.C. 1.14.18.1) that is caused by immobilization on glass beads and Celite(R). The degree of inactivation is dependent on the enzyme loading and the carrier's surface area. Addition of a sacrificial protein during the immobilization procedure offers a protective effect with increased residual activity on the basis of comparable enzyme loading.

引用

页码：471 / 476

页数：6

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