SPLIT GENE FOR MITOCHONDRIAL 24S RIBOSOMAL-RNA OF NEUROSPORA-CRASSA

被引：68

作者：

HAHN, U ^{[1
]}

LAZARUS, CM ^{[1
]}

LUNSDORF, H ^{[1
]}

KUNTZEL, H ^{[1
]}

机构：

[1] MAX PLANCK INST EXPTL MED, CHEM ABT, HERMANN REIN STR 3, D-3400 GOTTINGEN, FED REP GER

来源：

CELL | 1979年 / 17卷 / 01期

关键词：

D O I：

10.1016/0092-8674(79)90307-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The 60 kb [kilobase] circular mitochondrial genome of N. crassa contains a single transcription unit for 17S and 24S rRNA mapping within the largest Eco RI fragment E1 (19.6 kb). This fragment was isolated from uncloned mitochondrial DNA and further analyzed by cleavage with restriction endonucleases Hind II, Hind III, Bam HI, Pvu II and Bgl I, and by EM of rRNA/DNA hybrids. The resulting map shows a 2.3 kb intervening sequence interrupting the gene for 24S rRNA. The main part (2.7 kb) of this gene is separated from the 17S rRNA gene by a 5 kb segment which contains several transfer RNA genes. This segment is much longer than the putative 1 kb spacer sequence within the 32S precursor molecule for both rRNA, suggesting a second splicing event in that region.

引用

页码：191 / 200

页数：10

共 40 条

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