ESTROGEN-RECEPTOR IN HAMSTER-KIDNEY DURING ESTROGEN-INDUCED RENAL TUMORIGENESIS

被引:9
作者
ANDERSON, NS [1 ]
DAVID, Y [1 ]
FANESTIL, DD [1 ]
机构
[1] UNIV CALIF SAN DIEGO, DEPT MED, M013, LA JOLLA, CA 92093 USA
基金
美国国家卫生研究院;
关键词
D O I
10.1016/0022-4731(79)90225-5
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Specific binding of [3H]-estradiol to isolated cytosol and to nuclei in tissue slices was determined in hamster kidney before and during estrogen-induced renal tumorigenesis and in the kidney of normal rats, a species which does not develop the tumor. At 10-9M [3H]-estradiol, cytosol from normal hamster kidney bound 6.48 ± 0.3 × 10-15 mol/mg protein, which was significantly less than the 15.8 ± 1.1 × 10-15mol/mg bound by the rat. The apparent dissociation constant for estradiol was not significantly different (0.50 ± 0.08 nM in hamster and 0.66 ± 0.14 nM in rat). Cytosol binding increased after hamsters were implanted with estrogen pellets for 6 months (9.23 ± 0.2 × 10-15 mol/mg) or had developed tumors (13.7 ± 3.5 × 10-15 mol/mg). In tissue slices, the amount of [3H]-estradiol translocated to nuclei was greater in the rat (21.8 ± 0.8 × 10-14mol/mg nuclear protein) than in the normal hamster (3.28 ± 0.96 × 10-14 mol/mg) and was greater in tumor-bearing hamsters (6.70 ± 0.86 × 10-15 mol/mg DNA) than in normal hamsters (3.19 ± 0.63 × 10-15mol/mg DNA). We conclude: (1) the kidney of the tumor prone hamster does not contain an estrogen receptor of excessive quantity or unusual affinity and (2) the estrogen-receptor complex in estrogen-induced tumors can be translocated to cellular nuclei. © 1979.
引用
收藏
页码:123 / 128
页数:6
相关论文
共 17 条
[1]   CORTICOID RECEPTORS IN RAT-BRAIN - EVIDENCE FOR AN ALDOSTERONE RECEPTOR [J].
ANDERSON, NS ;
FANESTIL, DD .
ENDOCRINOLOGY, 1976, 98 (03) :676-684
[2]  
ANDERSON NS, 1976, FED PROC, V35, P568
[3]   EFFECT OF ANTIESTROGEN NAFOXIDINE ON OCCURRENCE OF ESTROGEN-DEPENDENT RENAL TUMORS IN HAMSTER [J].
ANTONIO, P ;
GABALDON, M ;
LACOMBA, T ;
JUAN, A .
HORMONE AND METABOLIC RESEARCH, 1974, 6 (06) :522-524
[5]   ESTRADIOL RENAL RECEPTOR MOLECULES AND ESTRADIOL-DEPENDENT ANTINATRIURESIS [J].
DEVRIES, JR ;
LUDENS, JH ;
FANESTIL, DD .
KIDNEY INTERNATIONAL, 1972, 2 (02) :95-+
[6]  
FERGUSSON JD, 1972, ENDOCRINE THERAPY MA, P237
[7]   HORMONALLY INDUCED RENAL NEOPLASIA IN MALE SYRIAN-HAMSTER AND INHIBITORY EFFECT OF 2-BROMO-ALPHA-ERGOCRYPTINE METHANESULFONATE [J].
HAMILTON, JM ;
FLAKS, A ;
SALUJA, PG ;
MAGUIRE, S .
JOURNAL OF THE NATIONAL CANCER INSTITUTE, 1975, 54 (06) :1385-1400
[8]  
KIRKMAN HADLEY, 1959, NAIL CANCER INST MONOGR, V1, P1
[9]   ESTROGEN BINDING-PROTEIN IN RENAL CYTOSOL OF INTACT, CASTRATED AND ESTROGENIZED GOLDEN-HAMSTERS [J].
LI, JJ ;
TALLEY, DJ ;
LI, SA ;
VILLEE, CA .
ENDOCRINOLOGY, 1974, 95 (04) :1134-1141
[10]  
LI JJ, 1976, CANCER RES, V36, P1127