A MODIFIED METHOD FOR CHONDROSULFATASE ASSAY

被引：218
作者：
KAWAI, Y
SENO, N
ANNO, K
机构：
[1] Department of Chemistry, Faculty of Science, Ochanomizu University, Bunkyo-ku, Tokyo
来源：
ANALYTICAL BIOCHEMISTRY | 1969年 / 32卷 / 02期
关键词：
D O I：
10.1016/0003-2697(69)90091-8
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The turbidimetric method of Dodgson and Price has been modified for chondrosulfatase assay. The method involves removal of undegraded chondroitin sulfates with cetylpyridinium chloride, modification of the barium chloride-gelatin reagent, and substitution of 0.2N hydrochloric acid for the 3% trichloroacetic acid of the original method. By using this modified method, the presence of chondrosulfatase activity was demonstrated in the liver of the squid, Ommastrephes sloani pacificus. © 1969.
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页码：314 / &
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