MALATHION EXPOSED SEEDS OF VIGNA-SINENSIS CHANGE IN THE ACTIVITY OF PLASMA-MEMBRANE BOUND MONO-VALENT ION STIMULATED ATPASE

被引:3
作者
CHAKRABORTI, S
DAS, KK
BANERJEE, SK
机构
[1] Department of Biochemistry, University College of Science, Calcutta-
关键词
D O I
10.1080/00207237908737396
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Plasma membrane bound ATPase of Vigna sinensis (L) is activated by Mg+2 and is further stimulated by monovalent cations like Na+/K+, at a definite pH and substrate concentration. The true substrate for the enzyme was Mg++ -ATP. Ca++ cannot replace Mg++ so far as the activation of this enzyme is concerned. Activation does not alter much when treated with ouabain. These results support the concept that cation transport at the plasma membrane of root cells of Vigna sinensis (L) is coupled to a cation-activated ATPase. Malathion (O, O-dimethyl phosphorodithioate of diethyl mercapto succinate), an organo phosphorus insecticide, activates these enzymes at a concentration of 400 ppm. This increase in activity may be associated with some alteration in the membrane structure, and stimulation by malathion has been found to be of non-competitive in nature when the divalent and monovalent ions are included in the system. © 1979, Taylor & Francis Group, LLC. All rights reserved.
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页码:217 / 221
页数:5
相关论文
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