OVERCOMING THE 2-CELL BLOCK BY MODIFYING STANDARD-COMPONENTS IN A MOUSE EMBRYO CULTURE-MEDIUM

被引：98

作者：

LAWITTS, JA ^{[1
]}

BIGGERS, JD ^{[1
]}

机构：

[1] HARVARD UNIV, SCH MED, HUMAN REPROD & REPROD BIOL LAB, 25 SHATTUCK ST, BOSTON, MA 02115 USA

来源：

BIOLOGY OF REPRODUCTION | 1991年 / 45卷 / 02期

关键词：

D O I：

10.1095/biolreprod45.2.245

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The 2-cell block may be caused by inappropriate concentrations of commonly used constituents of embryo culture media. Almost all zygotes obtained by fertilizing CF1 ova with hybrid B6D2F1/CrlBR sperm did not develop beyond the 2-cell stage when cultured in Whittingham's medium M16. This 2-cell block was overcome by lowering the concentrations of NaCl, KCl, KH2PO4, glucose, and pyruvate, either individually or in combination. The effects of changing the concentration of either NaCl or KCl depend on the concentration of NaHCO3 in the medium. Although a high percentage of embryos grew to the 4-cell stage in several media with lowered concentrations of certain components, the media are not optimal for complete preimplantation embryo development since the yield of blastocysts is low.

引用

页码：245 / 251

页数：7

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