PROTEIN KINASE-C(19-31) PSEUDOSUBSTRATE INHIBITION OF INSULIN ACTION IN RAT ADIPOCYTES

被引：10
|
作者：
STANDAERT, ML
SASSE, J
COOPER, DR
FARESE, RV
机构：
[1] UNIV S FLORIDA,DEPT INTERNAL MED,TAMPA,FL 33612
[2] UNIV S FLORIDA,DEPT BIOCHEM MOLEC BIOL,TAMPA,FL 33612
来源：
FEBS LETTERS | 1991年 / 282卷 / 01期
关键词：
INSULIN; PROTEIN KINASE-C; HEXOSE UPTAKE; ADIPOCYTE;
D O I：
10.1016/0014-5793(91)80463-D
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Treatment of intact adipocytes with the autoregulatory PKC pseudosubstrate PKC(19-31) inhibited insulin-stimulated hexose uptake and lipogenesis, with no effect on basal values. The effect was dose-dependent with respective IC50 values of 30-mu-M and 600-mu-M for insulin-stimulated hexose uptake in electroporated and intact adipocytes. These studies indicate that PKC may play a role in the mediation of insulin action in adipocytes.
引用
收藏
页码：139 / 142
页数：4
相关论文
共 50 条
  • [31] INSULIN INCREASES THE SYNTHESIS OF PHOSPHOLIPID AND DIACYLGLYCEROL AND PROTEIN KINASE-C ACTIVITY IN RAT HEPATOCYTES
    COOPER, DR
    HERNANDEZ, H
    KUO, JY
    FARESE, RV
    ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1990, 276 (02) : 486 - 494
  • [32] SITES OF ACTION OF PROTEIN KINASE-C ON SECRETORY ACTIVITY IN RAT PARIETAL-CELLS
    HATT, JF
    HANSON, PJ
    AMERICAN JOURNAL OF PHYSIOLOGY, 1989, 256 (01): : G129 - G138
  • [33] EFFECT OF PROTEIN KINASE-C INHIBITION ON NEUTROPHIL ADHERENCE
    WEBSTER, RO
    KOEPNICK, SL
    FASEB JOURNAL, 1988, 2 (06): : A1606 - A1606
  • [34] INHIBITION OF PROTEIN KINASE-C BY THE LIPOPHOSPHOGLYCAN OF LEISHMANIA PARASITES
    MCNEELY, TB
    TURCO, SJ
    GLYCOCONJUGATE JOURNAL, 1988, 5 (03) : 307 - 307
  • [35] MODES OF INHIBITION OF PROTEIN KINASE-C BY TRIPHENYLACRYLONITRILE ANTIESTROGENS
    BIGNON, E
    OGITA, K
    KISHIMOTO, A
    GILBERT, J
    ABECASSIS, J
    MIQUEL, JF
    NISHIZUKA, Y
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 163 (03) : 1377 - 1383
  • [36] INHIBITION OF PROTEIN KINASE-C ACTIVITY BY PHOTOACTIVATED PSORALENS
    MA, W
    LASKIN, JD
    JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1990, 94 (04) : 552 - 552
  • [37] CARBON TETRACHLORIDE-INDUCED INHIBITION OF PROTEIN KINASE-C IN ISOLATED RAT HEPATOCYTES
    POLI, G
    ALBANO, E
    DIANZANI, MU
    MELLONI, E
    PONTREMOLI, S
    MARINARI, UM
    PRONZATO, MA
    COTTALASSO, D
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1988, 153 (02) : 591 - 597
  • [38] PROTEIN KINASE-C INHIBITION BY ETHER LIPID ANALOGS
    DANIEL, LW
    NOSEDA, A
    MODEST, EJ
    MARASCO, CJ
    MORRISNATSCHKE, S
    MARX, MH
    ISHAQ, KS
    PIANTADOSI, C
    PROCEEDINGS OF THE AMERICAN ASSOCIATION FOR CANCER RESEARCH, 1988, 29 : 321 - 321
  • [39] INHIBITION OF PROTEIN KINASE-C ACTIVITY BY PHOTOACTIVATED PSORALENS
    MA, W
    LASKIN, JD
    CLINICAL RESEARCH, 1990, 38 (02): : A666 - A666
  • [40] PHORBOL ESTER AND INSULIN STIMULATE PROTEIN-KINASE-C ISOFORMS IN RAT ADIPOCYTES
    ISHIZUKA, T
    YAMAMOTO, M
    KAJITA, K
    NAGASHIMA, T
    TANIGUCHI, O
    WADA, H
    ITAYA, S
    YASUDA, K
    DIABETES RESEARCH AND CLINICAL PRACTICE, 1994, 26 (02) : 91 - 99
12345