PROPERTIES OF FUNGAL PROTEASE INHIBITORS FROM THE INTEGUMENT AND HEMOLYMPH OF THE SILKWORM AND EFFECT OF AN INHIBITOR ON THE FUNGAL GROWTH

被引：31

作者：

YOSHIDA, S ^{[1
]}

YAMASHITA, M ^{[1
]}

YONEHARA, S ^{[1
]}

EGUCHI, M ^{[1
]}

机构：

[1] KYOTO INST TECHNOL,DEPT APPL BIOL,KYOTO 606,JAPAN

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 1990年 / 95卷 / 03期

关键词：

D O I：

10.1016/0305-0491(90)90021-K

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. 1. Protein inhibitors in the haemolymph and integument of the silkworm, Bombyx mori were studied using fungal proteases from Aspergillus melleus and Beauveria bassiana. 2. 2. The polyacrylamide gel electrophoresis revealed inhibitor bands A, D, E and F against both proteases in the haemolymph, whereas band D was undiscernible in the integument. 3. 3. DEAE-Sephacel column chromatography showed inhibitor fractions in the haemolymph corresponding to electrophoretic bands and a main peak, F, in the integument was seen against the above proteases. 4. 4. The purified inhibitor F was found to inhibit the germination of conidia and germ tube development of B. bassiana. © 1990.

引用

页码：559 / 564

页数：6

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