A NOVEL CATIONIC LIPOSOME REAGENT FOR EFFICIENT TRANSFECTION OF MAMMALIAN-CELLS

被引：830

作者：

GAO, X ^{[1
]}

HUANG, L ^{[1
]}

机构：

[1] UNIV TENNESSEE,DEPT BIOCHEM,KNOXVILLE,TN 37996

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 179卷 / 01期

关键词：

D O I：

10.1016/0006-291X(91)91366-K

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel cationic derivative of cholesterol, 3β[N-(N′,N′-dimethylaminoethane)-carbamoyl] cholesterol (DC-Chol), has been synthesized and used to prepare sonicated liposomes with dioleoylphosphatidylethanolamine. This novel cationic liposome reagent facilitates efficient DNA mediated transfection in A431 human epidermoid carcinoma cells, A549 human lung carcinoma cells, L929 mouse fibroblast cells, and YPT minipig primary endothelial cells. The activity was greater than that of a commercial reagent, Lipofectin, and was approximately 4-fold less toxic than Lipofectin when assayed with A431 cells. The reagent is easy to synthesize and stable for at least 6 weeks. © 1991.

引用

页码：280 / 285

页数：6

共 12 条

[1]

BRINGHAM KL, 1989, AM J RESP CELL MOL B, V1, P95

[2] LIPOFECTION - A HIGHLY EFFICIENT, LIPID-MEDIATED DNA-TRANSFECTION PROCEDURE [J].