A NOVEL 1745-DALTON PYROGLUTAMYL PEPTIDE DERIVED FROM CHROMOGRANIN-B IS IN THE BOVINE ADRENOMEDULLARY CHROMAFFIN VESICLE

被引：44
作者：
FLANAGAN, T
TAYLOR, L
POULTER, L
VIVEROS, OH
DILIBERTO, EJ
机构：
[1] UNIV CALIF SAN FRANCISCO, DIV ORGAN CHEM, SAN FRANCISCO, CA 94143 USA
[2] UNIV CALIF SAN FRANCISCO, SCH PHARM, SAN FRANCISCO, CA 94143 USA
来源：
CELLULAR AND MOLECULAR NEUROBIOLOGY | 1990年 / 10卷 / 04期
关键词：
ADRENAL GLAND; CHROMAFFIN VESICLES; CHROMOGRANIN-B; FAST ATOM BOMBARDMENT MASS SPECTROMETRY; SECRETOGRANIN-I; SECRETORY PEPTIDE; POSTTRANSLATIONAL PROCESSING; PYROGLUTAMATE; PYRROLIDONE CARBOXYLIC ACID;
D O I：
10.1007/BF00712845
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
1. Following the recent demonstration of a glutaminyl cyclase activity localized in adrenomedullary chromaffin vesicles, an assay was developed to isolate and characterize posttranslationally modified peptides from this tissue which contain pyroglutamate. This assay consisted of spectrometric identification of peptides before and after enzymatic removal of pyroglutamyl residues. 2. Using this procedure, a pyroglutamyl peptide (BAM-1745) was isolated and sequenced and was shown to be a significant component of adrenomedullary secretory vesicles. 3. A computer search through the Swiss-Prot protein sequence database revealed a 93% identity of BAM-1745 and a fragment of human chromogranin B (Gln580-Tyr593).
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页码：507 / 523
页数：17
相关论文
共 59 条
  • [1] ANDREWS PC, 1989, METHOD ENZYMOL, V168, P72
  • [2] FAST ATOM BOMBARDMENT OF SOLIDS (FAB) - A NEW ION-SOURCE FOR MASS-SPECTROMETRY
    BARBER, M
    BORDOLI, RS
    SEDGWICK, RD
    TYLER, AN
    [J]. JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS, 1981, (07) : 325 - 327
  • [3] NEUROPEPTIDE-Y IS LOCALIZED TOGETHER WITH ENKEPHALINS IN ADRENERGIC GRANULES OF BOVINE ADRENAL-MEDULLA
    BASTIAENSEN, E
    DEBLOCK, J
    DEPOTTER, WP
    [J]. NEUROSCIENCE, 1988, 25 (02) : 679 - 686
  • [4] A SPECTROPHOTOMETRIC ASSAY FOR GLUTAMINYL-PEPTIDE CYCLIZING ENZYMES
    BATEMAN, RC
    [J]. JOURNAL OF NEUROSCIENCE METHODS, 1989, 30 (01) : 23 - 28
  • [5] THE PRIMARY STRUCTURE OF BOVINE CHROMOGRANIN-A - A REPRESENTATIVE OF A CLASS OF ACIDIC SECRETORY PROTEINS COMMON TO A VARIETY OF PEPTIDERGIC CELLS
    BENEDUM, UM
    BAEUERLE, PA
    KONECKI, DS
    FRANK, R
    POWELL, J
    MALLET, J
    HUTTNER, WB
    [J]. EMBO JOURNAL, 1986, 5 (07) : 1495 - 1502
  • [6] THE PRIMARY STRUCTURE OF HUMAN SECRETOGRANIN-I (CHROMOGRANIN-B) - COMPARISON WITH CHROMOGRANIN-A REVEALS HOMOLOGOUS TERMINAL DOMAINS AND A LARGE INTERVENING VARIABLE REGION
    BENEDUM, UM
    LAMOUROUX, A
    KONECKI, DS
    ROSA, P
    HILLE, A
    BAEUERLE, PA
    FRANK, R
    LOTTSPEICH, F
    MALLET, J
    HUTTNER, WB
    [J]. EMBO JOURNAL, 1987, 6 (05) : 1203 - 1211
  • [7] CHROMOGRANIN-B (SECRETOGRANIN-I), A PUTATIVE PRECURSOR OF 2 NOVEL PITUITARY PEPTIDES THROUGH PROCESSING AT PAIRED BASIC RESIDUES
    BENJANNET, S
    LEDUC, R
    ADROUCHE, N
    FALGUEYRET, JP
    MARCINKIEWICZ, M
    SEIDAH, NG
    MBIKAY, M
    LAZURE, C
    CHRETIEN, M
    [J]. FEBS LETTERS, 1987, 224 (01): : 142 - 148
  • [8] CHARACTERIZATION BY TANDEM MASS-SPECTROMETRY OF STRUCTURAL MODIFICATIONS IN PROTEINS
    BIEMANN, K
    SCOBLE, HA
    [J]. SCIENCE, 1987, 237 (4818) : 992 - 998
  • [9] CORTICOTROPIN-RELEASING FACTOR IN THE ADRENAL-MEDULLA
    BRUHN, TO
    ENGELAND, WC
    ANTHONY, ELP
    GANN, DS
    JACKSON, IMD
    [J]. ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1987, 512 : 115 - 128
  • [10] BUSBY WH, 1987, J BIOL CHEM, V262, P8532
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