PRAZOSIN DETERMINATION BY HIGH-PRESSURE LIQUID-CHROMATOGRAPHY USING FLUORESCENCE DETECTION

被引：60

作者：

YEE, YG

RUBIN, PC

MEFFIN, P

机构：

[1] STANFORD UNIV,MED CTR,DEPT MED,DIV CARDIOL,PHARMACOKINET LAB,STANFORD,CA 94305

[2] STANFORD UNIV,MED CTR,DEPT MED,DIV CLIN PHARMACOL,STANFORD,CA 94305

来源：

JOURNAL OF CHROMATOGRAPHY | 1979年 / 172卷 / APR期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/S0021-9673(00)90967-1

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A high-pressure liquid chromatographic procedure using fluorescence detection for the analysis of prazosin in whole blood and plasma is described. It employs a simple and rapid method of sample preparation. Prazosin and an internal standard are chromatographed as ion-pairs with pentane sulfonic acid. The method is sensitive and reproducible with accurate detection as low as 0.2 ng/ml in whole blood and 0.5 ng/ml in plasma with a coefficient of variation of 5.6% and 5.4%, respectively. If propranolol or quinidine is present, modifications of chromatographic conditions are used which separate prazosin from these two drugs. Prazosin is stable when frozen for a long period or refrigerated for short periods. © 1979.

引用

页码：313 / 318

页数：6

共 5 条

[1] EFFICACY OF AMBULATORY SYSTEMIC VASODILATOR THERAPY WITH ORAL PRAZOSIN IN CHRONIC REFRACTORY HEART-FAILURE - CONCOMITANT RELIEF OF PULMONARY CONGESTION AND ELEVATION OF PUMP OUTPUT DEMONSTRATED BY IMPROVEMENTS IN SYMPTOMATOLOGY, EXERCISE TOLERANCE, HEMODYNAMICS AND ECHOCARDIOGRAPHY [J].