MOLECULAR CHARACTERIZATION OF 2 FORMS OF NONTOXIC-NONHEMAGGLUTININ COMPONENTS OF CLOSTRIDIUM-BOTULINUM TYPE-A PROGENITOR TOXINS

被引：32

作者：

FUJITA, R

FUJINAGA, Y

INOUE, K

NAKAJIMA, H

KUMON, H

OGUMA, K

机构：

[1] OKAYAMA UNIV, SCH MED, DEPT BACTERIOL, OKAYAMA 700, JAPAN

[2] OKAYAMA UNIV, SCH MED, DEPT UROL, OKAYAMA 700, JAPAN

来源：

FEBS LETTERS | 1995年 / 376卷 / 1-2期

关键词：

CLOSTRIDIUM BOTULINUM; BOTULINUM TOXIN; NEUROTOXIN; PROGENITOR TOXIN; NONTOXIC-NONHEMAGGLUTININ; PCR;

D O I：

10.1016/0014-5793(95)01241-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The entire sequences of the type A nontoxic-nonhemagglutinin gene and an adjacent open reading frame designated as orf 22-a, which are located between the neurotoxin and the HA-35 genes were determined, SDS-PAGE and N-terminal amino acid sequence analyses of the purified type A progenitor toxins (12S, 16S and 19S) indicate that the nontoxic-nonhemagglutinins of 16S and 19S are single peptides of approximately 120k, but that of 12S has a cleavage at the site between Pro-144 and Phe-145 of this protein.

引用

页码：41 / 44

页数：4

共 16 条

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