SUPPRESSED DNA-REPAIR CAPACITY OF PERIPHERAL LYMPHOCYTES IN PREGNANT-WOMEN

The ability to repair potentially carcinogenic lesions was measured for peripheral lymphocytes isolated from women in the first, second and third trimesters of pregnancy and 6 weeks post-partum. Freshly isolated lymphocytes were damaged with 254 nm ultraviolet radiation and allowed to perform repair in their autologous plasma DNA repair capacity during pregnancy was over 50% lower than that measured 6 weeks post-partum. No significant difference in repair capacity was observed among the three trimesters. Mitogen-stimulated DNA synthesis was also measured for cells cultured in their autologous plasma. There was no significant difference in thymidine incorporation between cells collected during pregnancy (1.02 +/- 0.17 cpm/10(6) cells) and cells collected 6 weeks postpartum (1.45 +/- 0.94 cpm/10(6) cells). The results are, however, consistent with alterations in the immune response during pregnancy.