IN-VIVO REPRESSION BY A SITE-SPECIFIC DNA-BINDING PROTEIN DESIGNED AGAINST AN ONCOGENIC SEQUENCE

被引：238
作者：
CHOO, Y
SANCHEZGARCIA, I
KLUG, A
机构：
[1] Medical Research Council, Laboratory of Molecular Biology, Cambridge CB2 2QH, Hills Road
来源：
NATURE | 1994年 / 372卷 / 6507期
关键词：
D O I：
10.1038/372642a0
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
A DNA-binding peptide comprising three zinc-fingers has been engineered to bind specifically to a unique nine-base-pair region of a BCR-ABL fusion oncogene in preference to the parent genomic sequences. Binding to the target oncogene in chromosomal DNA is possible In transformed cells in culture, and results in blockage of transcription. Consequently, murine cells rendered independent of growth factors by the action of the oncogene revert to factor dependence upon transient transfection with a vector expressing the peptide.
引用
收藏
页码：642 / 645
页数：4
相关论文
共 37 条
[1]   TRANSLOCATION OF C-ABL ONCOGENE CORRELATES WITH THE PRESENCE OF A PHILADELPHIA-CHROMOSOME IN CHRONIC MYELOCYTIC-LEUKEMIA [J].
BARTRAM, CR ;
DEKLEIN, A ;
HAGEMEIJER, A ;
VANAGTHOVEN, T ;
VANKESSEL, AG ;
BOOTSMA, D ;
GROSVELD, G ;
FERGUSONSMITH, MA ;
DAVIES, T ;
STONE, M ;
HEISTERKAMP, N ;
STEPHENSON, JR ;
GROFFEN, J .
NATURE, 1983, 306 (5940) :277-280
[2]   PROPOSED STRUCTURE FOR THE ZINC-BINDING DOMAINS FROM TRANSCRIPTION FACTOR-IIIA AND RELATED PROTEINS [J].
BERG, JM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (01) :99-102
[3]   A ROLE IN DNA-BINDING FOR THE LINKER SEQUENCES OF THE 1ST 3 ZINC FINGERS OF TFIIIA [J].
CHOO, Y ;
KLUG, A .
NUCLEIC ACIDS RESEARCH, 1993, 21 (15) :3341-3346
[4]   SELECTION OF DNA-BINDING SITES FOR ZINC FINGERS USING RATIONALLY RANDOMIZED DNA REVEALS CODED INTERACTIONS [J].
CHOO, Y ;
KLUG, A .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (23) :11168-11172
[5]   TOWARD A CODE FOR THE INTERACTIONS OF ZINC FINGERS WITH DNA - SELECTION OF RANDOMIZED FINGERS DISPLAYED ON PHAGE [J].
CHOO, Y ;
KLUG, A .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (23) :11163-11167
[6]   A GENE ACTIVATED IN MOUSE 3T3-CELLS BY SERUM GROWTH-FACTORS ENCODES A PROTEIN WITH ZINC FINGER SEQUENCES [J].
CHRISTY, BA ;
LAU, LF ;
NATHANS, D .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (21) :7857-7861
[7]  
COONEY M, 1988, SCIENCE, V245, P725
[8]   TRANSFORMATION OF AN INTERLEUKIN-3-DEPENDENT HEMATOPOIETIC-CELL LINE BY THE CHRONIC MYELOGENOUS LEUKEMIA-SPECIFIC P210BER/ABL PROTEIN [J].
DALEY, GQ ;
BALTIMORE, D .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (23) :9312-9316
[9]   TOWARD RULES RELATING ZINC FINGER PROTEIN SEQUENCES AND DNA-BINDING SITE PREFERENCES [J].
DESJARLAIS, JR ;
BERG, JM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (16) :7345-7349
[10]   FIREFLY LUCIFERASE GENE - STRUCTURE AND EXPRESSION IN MAMMALIAN-CELLS [J].
DEWET, JR ;
WOOD, KV ;
DELUCA, M ;
HELINSKI, DR ;
SUBRAMANI, S .
MOLECULAR AND CELLULAR BIOLOGY, 1987, 7 (02) :725-737
1234