MULTIPLE GENES OF DELTA-ENDOTOXINS OF BACILLUS-THURINGIENSIS SSP GALLERIAE

delta-Endotoxin genes cryIAb7, cryIG, and cryIX of Bacillus thuringiensis ssp. galleriae were cloned. The genes were thoroughly studied; their expression in Escherichia coli cells was analyzed. Partial sequencing and restriction endonuclease mapping revealed identity of 3' ends of cryIX and cryIG genes; each of the toxin genes possesses a unique 5' sequence. Sequencing of the 5' part of cryIX did not reveal any characteristic elements of the translation initiation site; this fact indicates that in natural conditions this gene does not function. CryIG and cryIX form a cluster. The absence of promoter-like structure from the 5' part of cryIG lets us hypothesize that both genes are transcribed from one and the same promoter. The 3' parts of cryIX and cryIG genes 2000 bp long are highly homologous; one may suggest that recombination events took place in the evolution of these genes