ANALYSIS OF LARCH ARABINOGALACTAN BY HIGH-PERFORMANCE SIZE-EXCLUSION CHROMATOGRAPHY

被引：12
|
作者：
EREMEEVA, TE
BYKOVA, TO
机构：
[1] Cellulose Laboratory, Institute of Wood Chemistry, Latvian Academy of Science, Riga, 226006
来源：
CARBOHYDRATE POLYMERS | 1992年 / 18卷 / 03期
关键词：
D O I：
10.1016/0144-8617(92)90066-Y
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
The elution behaviour of larch arabinogalactan (AGal) during size-exclusion chromatography (SEC) in aqueous solutions, using a Separon S HEMA Bio 1000 column, has been investigated. It is established that the polydispersity of AGal in pure water is caused by its chemical heterogeneity. It is found that to obtain the correct molecular mass distribution (MMD) of AGal, SEC measurements should be performed with an 0-4 M acetate buffer of pH 5.5 as the eluent to prevent polyelectrolyte effects. From the SEC data the larch AGal is characterized by a nearly Gaussian MMD, a narrow polydispersity (M(w)/M(n) approximately 1.1-1.4) and an M(w)BAR of ca. 10 000.
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页码：217 / 219
页数：3
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