DEVELOPMENT AND VALIDATION OF A GC-MS METHOD FOR THE SIMULTANEOUS QUANTITATION OF ZEARALENONE AND ITS METABOLITES IN WATER

The method of simultaneous determination of zearalenone and its metabolites in water with use of GC-MS chromatography was evaluated. The solid phase extraction SPE was used for analytes separation from water and as an enrichment method. The influence of compounds concentration and water sample volume on analytes recovery was investigated. The chromatographic analysis was preceded by conversion of compounds to trimethylsilyl ethers derivatives. The developed SPE-derivatization-GC/MS method allow to separate quaternary mixture of mycoestrogens with the quantitative analysis in water, when their concentration is greater than 0.2-0.5 ng/dm(3). The repeatability of the results was from 1 to 8 %. The recovery of compounds exceeded 60 % for samples contains from 50 to 200 ng/dm(3) of mycotoxins. The concentration of analytes and volume of the sample (100-500 cm(3)) did not have an influence on the extraction output. The developed method can be applied to analyze water samples containing mycoestrogens at the level of ng/dm(3).