NETROPSIN, A DNA-BINDING OLIGOPEPTIDE STRUCTURAL AND BINDING STUDIES

被引：124

作者：

BERMAN, HM

NEIDLE, S

ZIMMER, C

THRUM, H

机构：

[1] UNIV LONDON,KINGS COLL,DEPT BIOPHYS,LONDON WC2B 5RL,ENGLAND

[2] AKAD WISSENSCH DDR,MOLEK BIOL & MED FORSCHUNGSZENTRUM,ZENT INST MIKROBIOL & EXPTL THERAPIE,DDR-69 JENA,GER DEM REP

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 561卷 / 01期

基金：

美国国家卫生研究院;

关键词：

(Structure); Antibiotic; DNA-binding oligopeptide; Netropsin; X-ray crystallography;

D O I：

10.1016/0005-2787(79)90496-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The crystal structure of netropsin, an oligopeptide which binds to DNA, has been determined. The molecule is bowed with the amide groups on the concave side, and the carbonyl and methyl groups on the convex side. The amide groups participate in extensive hydrogen bonding with water molecules; the charged amino end groups interact with the sulfate anions. Binding of netropsin to poly(dA) · poly(dT) under conditions of different ionic strength was also studied. Utilizing the crystallographic as well as the binding data, it is possible to build a model which explains the specificity of this antibiotic. © 1979.

引用

收藏

页码：124 / 131

页数：8

相关论文

共 12 条

[1] CONFORMATION DEPENDENT BINDING OF NETROPSIN AND DISTAMYCIN TO DNA AND DNA MODEL POLYMERS [J].

LUCK, G ;

TRIEBEL, H ;

WARING, M ;

ZIMMER, C .

NUCLEIC ACIDS RESEARCH, 1974, 1 (03) :503-530

[2] USE OF NETROPSIN WITH CSCL GRADIENTS FOR ANALYSIS OF DNA AND ITS APPLICATION TO RESTRICTION NUCLEASE FRAGMENTS OF RIBOSOMAL DNA FROM PHYSARUM-POLYCEPHALUM [J].

MATTHEWS, HR ;

JOHNSON, EM ;

STEER, WM ;

BRADBURY, EM ;

ALLFREY, VG .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1978, 82 (02) :569-576

[3] THEORETICAL CALCULATIONS OF HELIX-COIL TRANSITION OF DNA IN PRESENCE OF LARGE, COOPERATIVELY BINDING LIGANDS [J].

MCGHEE, JD .

BIOPOLYMERS, 1976, 15 (07) :1345-1375

[4] NETROPSIN-POLY(DA-DT) COMPLEX IN SOLUTION - STRUCTURE AND DYNAMICS OF ANTIBIOTIC-FREE BASE PAIR REGIONS AND THOSE CENTERED ON BOUND NETROPSIN [J].

PATEL, DJ ;

CANUEL, LL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (12) :5207-5211

[5] RNA DOUBLE-HELICAL FRAGMENTS AT ATOMIC RESOLUTION - .1. CRYSTAL AND MOLECULAR-STRUCTURE OF SODIUM ADENYLYL-3',5'-URIDINE HEXAHYDRATE [J].

SEEMAN, NC ;

ROSENBERG, JM ;

SUDDATH, FL ;

PARKKIM, JJ ;

RICH, A .

JOURNAL OF MOLECULAR BIOLOGY, 1976, 104 (01) :109-144

[6] SEQUENCE-SPECIFIC RECOGNITION OF DOUBLE HELICAL NUCLEIC-ACIDS BY PROTEINS [J].

SEEMAN, NC ;

ROSENBERG, JM ;

RICH, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1976, 73 (03) :804-808

[7] (DA.DT)-DEPENDENT INACTIVATION OF DNA TEMPLATE PROPERTIES BY INTERACTION WITH NETROPSIN AND DISTAMYCIN-A [J].

WAHNERT, U ;

ZIMMER, C ;

LUCK, G ;

PITRA, C .

NUCLEIC ACIDS RESEARCH, 1975, 2 (03) :391-404

[8]

WARTELL RM, 1974, J BIOL CHEM, V249, P6719

[9] BINDING OF NETROPSIN TO DNA AND SYNTHETIC POLYNUCLEOTIDES [J].

ZASEDATELEV, AS ;

THRUM, H ;

GURSKY, GV ;

ZIMMER, C .

MOLECULAR BIOLOGY REPORTS, 1974, 1 (06) :337-342

[10]

ZASEDATELEV AS, 1976, DOKL AKAD NAUK SSSR+, V231, P1006