Cytotoxic Potential and Molecular Characterization of Fungal Endophytes from Selected High Value Medicinal Plants of the Kashmir Valley - India

被引：2

作者：

Dar, R. A. ^{[1
]}

Qazi, P. H. ^{[1
]}

Saba, I. ^{[1
]}

Rather, S. A. ^{[1
]}

Wani, Z. A. ^{[2
]}

Qazi, A. K. ^{[2
]}

Shiekh, A. A. ^{[1
]}

Manzoor, A. ^{[1
]}

Hamid, A. ^{[2
]}

Modae, D. M. ^{[2
]}

机构：

[1] CSIR Indian Inst Integrat Med, Div Biotechnol, Sanatnagar Srinagar 190005, Kashmir, India

[2] CSIR Indian Inst Integrat Med, Canc Pharmacol Div, Jammu, India

来源：

DRUG RESEARCH | 2016年 / 66卷 / 03期

关键词：

ehrlich ascites carcinoma; SRB; secondary metabolites;

D O I：

10.1055/s-0035-1550042

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

The present study explores the fungal endophytes from selected high value medicinal plants to check their activities at in-vitro and in-vivo level. The in-vitro cytotoxicity of selected endophytes revealed potent growth inhibition against human cancer cell lines of leukemia (THP-1), lung (A549), prostate (PC-3), colon (Caco-2), neuroblastoma (IMR-32) and breast (MCF-7) at a concentration of 100 mu g/ml. Among them the endophytic strains i.e., IIIM2, IIIM3, IIIM7 and IIIM8 showed most significant growth inhibition against colon (Caco-2), prostate (PC-3), lung (A549) and leukemia (THP-1) cancer cell lines. At the in-vivo level maximum (58.95 %) tumor growth inhibition was documented with the extract of IIIM2 against Ehrlich Ascites Carcinoma mouse modal. All the potent fungal endophytic strains were characterized using ITS 4 and ITS 5 region sequencing and phylogenetic analysis was ascertained among them. This paper confirms the 2 elite endophytic fungal strains, IIIM2 and IIIM8, have the potential to act as a source of new anticancer compounds.

引用

页码：121 / 125

页数：5

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