THE MDM-2 GENE IS INDUCED IN RESPONSE TO UV-LIGHT IN A P53-DEPENDENT MANNER

被引：265

作者：

PERRY, ME ^{[1
]}

PIETTE, J ^{[1
]}

ZAWADZKI, JA ^{[1
]}

HARVEY, D ^{[1
]}

LEVINE, AJ ^{[1
]}

机构：

[1] PRINCETON UNIV,DEPT MOLEC BIOL,PRINCETON,NJ 08544

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1993年 / 90卷 / 24期

关键词：

CELL CYCLE; TRANSCRIPTION;

D O I：

10.1073/pnas.90.24.11623

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Irradiation of mammalian cells with UV light results in a dose-dependent accumulation of the p53 tumor-suppressor gene product that is evident within 2 hr. UV treatment causes a dramatic increase in p53-specific transcriptional transactivation activity and an increase in expression of the p53 -responsive gene mdm-2. UV-stimulated mdm-2 expression is not directly correlated with the level of p53 protein in a cell because mdm-2 induction is delayed at high UV doses even though p53 levels rise almost immediately. Cells lacking p53 protein do not respond to UV by increasing their expression of mdm-2. The delayed induction of mdm-2 at high UV doses suggests that, in addition to p53 protein levels, other factors contribute to the regulation of mdm-2 expression following UV treatment. The time of induction of mdm-2 in cells treated with UV light correlates with recovery of normal rates of DNA synthesis, presumably after DNA repair. These data indicate a possible role for mdm-2 in cell cycle progression.

引用

页码：11623 / 11627

页数：5

共 22 条

[1] MDM2 EXPRESSION IS INDUCED BY WILD TYPE-P53 ACTIVITY [J].