AN ELECTROSPRAY-IONIZATION MASS-SPECTROMETRY STUDY OF COPPER ADDUCTS OF PROTONATED UBIQUITIN

被引：28
|
作者：
JIAO, CQ
FREISER, BS
CARR, SR
CASSADY, CJ
机构：
[1] MIAMI UNIV,DEPT CHEM,OXFORD,OH 45056
[2] PURDUE UNIV,DEPT CHEM,W LAFAYETTE,IN 47907
来源：
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY | 1995年 / 6卷 / 06期
关键词：
D O I：
10.1016/1044-0305(95)00198-M
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A study of the addition of Cu(II) to a ubiquitin electrospray solution shows that the copper ion in the ubiquitin remains doubly charged and displaces two protons on the protonated protein molecule. This observation indicates a chelating bond between the protein and the Cu(II) species. The addition of Cu(I) also was studied and significant intensity was observed for adducts with up to four Cu(I) species attached, with each Cu(I) bonded to one basic site on the protein.
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页码：521 / 524
页数：4
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