PROTECTIVE EFFECT OF 16,16-DIMETHYL PROSTAGLANDIN-E2 ON ISOLATED RAT HEPATOCYTES AGAINST COMPLEMENT-MEDIATED IMMUNE ATTACK

被引：1

作者：

KUREBAYASHI, Y ^{[1
]}

IKEDA, T ^{[1
]}

HONDA, Y ^{[1
]}

机构：

[1] DAIICHI PHARMACEUT CO LTD,EDOGAWA KU,TOKYO 134,JAPAN

来源：

DIGESTIVE DISEASES AND SCIENCES | 1992年 / 37卷 / 05期

关键词：

16,16-DIMETHYL PROSTAGLANDIN-E2; COMPLEMENT; ANTIGEN-ANTIBODY REACTION; HEPATOCYTOLYSIS; CYTOPROTECTION;

D O I：

10.1007/BF01296417

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

16,16-Dimethyl prostaglandin E2 was examined for its ability to inhibit complement-mediated in vitro hepatocytolysis by an antigen-antibody reaction. In the presence of fresh rat serum as a source of complement, 5-min culture of isolated rat hepatocytes with a monoclonal antibody against a rat liver-specific membranous antigen resulted in a marked, significant elevation in lactate dehydrogenase leakage into the culture medium. However, with heat-inactivated rat serum, such a reaction did not occur, indicating that the hepatocytolysis induced by the antibody was attributable to the membrane damaging action of complement activated by an antigen-antibody reaction. Pretreatment of the hepatocyte with 16,16-dimethyl prostaglandin E2 significantly suppressed the cytolytic reaction induced by the antibody in a concentration-dependent manner. These results show that 16,16-dimethyl prostaglandin E2 is capable of protecting isolated rat hepatocytes against the membrane-damaging insult of activated complement.

引用

页码：645 / 649

页数：5

共 16 条

[11]

RUWART MJ, 1985, IN VITRO CELL DEV, V8, P450

[12] ISOLATION AND PURIFICATION OF A RAT LIVER-SPECIFIC ANTIGEN FROM HEPATOCYTE MEMBRANE [J].

SATO, K ;

IKEDA, T ;

KAWAI, F ;

OSADA, Y .

JOURNAL OF BIOCHEMISTRY, 1987, 102 (04) :933-938

[13]

SATO K, 1985, ACTA PATHOL JAPON, V35, P1375

[14]

STACHURA J, 1980, FOL HISTOCH CYTOCHEM, V18, P311

[15]

STACHURA J, 1981, GASTROENTEROLOGY, V81, P211

[16] EFFECT OF 16,16 DIMETHYL PROSTAGLANDIN-E2 ON ASPIRIN INDUCED DAMAGE TO RAT GASTRIC EPITHELIAL-CELLS IN TISSUE-CULTURE [J].

TERANO, A ;

MACH, T ;

STACHURA, J ;

TARNAWSKI, A ;

IVEY, KJ .

GUT, 1984, 25 (01) :19-25

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