TRANSFORMATION OF THE INDUSTRIAL STRAIN OF ASPERGILLUS-ORYZAE WITH THE HOMOLOGOUS AMDS GENE AS A DOMINANT SELECTABLE MARKER

被引：34
作者：
GOMI, K
KITAMOTO, K
KUMAGAI, C
机构：
[1] National Research Institute of Brewing, Kita-ku, Tokyo, 114, 2-6-30, Takinogawa
来源：
JOURNAL OF FERMENTATION AND BIOENGINEERING | 1992年 / 74卷 / 06期
关键词：
D O I：
10.1016/0922-338X(92)90037-U
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A homologous transformation system has been developed for Aspergillus oryzae industrial strains using the A. oryzae acetamidase-encoding gene (amdS) as a dominant selectable marker. Although the transformation efficiency is relatively low (0.4-1 transformants per mug DNA), the transformants contained significantly high copy numbers of the plasmid integrated into the genome. In addition, the promoter of the amdS gene was replaced by the strong expression promoter of the alpha-amylase-encoding gene (amyB) from A. oryzae, thereby enhancing the selection of the transformants.
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页码：389 / 391
页数：3
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