MULTIPLE-DROP-ARRAY (MDA) TECHNIQUE FOR THE LARGE-SCALE TESTING OF CULTURE MEDIA VARIATIONS IN HANGING MICRODROP CULTURES OF SINGLE CELL SYSTEMS .1. TECHNIQUE

被引：19
作者：
POTRYKUS, I
HARMS, CT
LORZ, H
机构：
[1] Friedrich Miescher Institute, CH-4002 Basel
来源：
PLANT SCIENCE LETTERS | 1979年 / 14卷 / 03期
关键词：
D O I：
10.1016/0304-4211(79)90075-0
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
A novel approach to the rapid testing of numerous combinations of culture media components is presented. The 'multiple-drop-array' (MDA) technique uses 40 μl hanging drops as its experimental unit, arranged in a geometrical order according to the combination of factor gradients. It is suited for all single cell systems, including protoplasts, cells and microspores. The technique allows a small team to test 5000 media variations in 2- and 3-factor combinations with a single population of 2 × 106 cells within two days. © 1979.
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页码：231 / 235
页数：5
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