CHARACTERIZATION OF THE HIGHLY ABUNDANT POLYMORPHIC GC-RICH-REPETITIVE SEQUENCE (PGRS) PRESENT IN MYCOBACTERIUM-TUBERCULOSIS

被引：95

作者：

POULET, S ^{[1
]}

COLE, ST ^{[1
]}

机构：

[1] INST PASTEUR,UNITE GENET MOLEC BACTERIENNE,F-75724 PARIS 15,FRANCE

来源：

ARCHIVES OF MICROBIOLOGY | 1995年 / 163卷 / 02期

关键词：

REPETITIVE SEQUENCES; TRIPLET REPEATS; STRAIN TYPING; EPIDEMIOLOGY; TUBERCULOSIS;

D O I：

10.1007/BF00381781

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The polymorphic GC-rich repetitive sequence (PGRS) found on the chromosome of Mycobacterium tuberculosis was characterized by means of mapping, cloning and sequencing. PGRS was present in at least 26 loci and consisted of many tandem repeats of the consensus sequence CGGCGGCAA. As the core of the consensus motif was the tripler CGG, or CRR (where R is a purine), it seems likely that PGRS arose by means of triplet expansion, accounting for its polymorphism. Several copies of PGRS were linked to a conserved open reading frame. PGRS was used as the target sequence for the polymerase chain reaction in an attempt to develop a new typing technique.

引用

页码：87 / 95

页数：9

共 29 条

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