SEX IDENTIFICATION BY POLYMERASE CHAIN-REACTION OF ALPHA-SATELLITE IN AGED TISSUE SAMPLES

被引：13

作者：

FATTORINI, P

CACCIO, S

GUSTINCIH, S

FLORIAN, F

ALTAMURA, BM

GRAZIOSI, G

机构：

[1] UNIV TRIESTE,DIPARTIMENTO BIOL,VIA VALERIO 32,I-34127 TRIESTE,ITALY

[2] INT CTR GENET ENGN & BIOTECHNOL,TRIESTE,ITALY

[3] UNIV TRIESTE,IST MED LEGALE ASSICURAZIONI,I-34127 TRIESTE,ITALY

[4] UNIV PAVIA,DIPARTIMENTO GENET,I-27100 PAVIA,ITALY

来源：

ELECTROPHORESIS | 1993年 / 14卷 / 1-2期

关键词：

D O I：

10.1002/elps.1150140105

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In vitro amplification of the alphoid repeated sequences clustered in the centromeric regions of the human X and Y chromosomes was carried out. A modification of the amplification conditions reported by Witt and Erickson [1] gave clear amplification bands from 10 pg of genomic DNA template. Results were obtained from the following aged tissue samples: 1 muL three-year-old blood stain, one-year-old single hair roots, six-month-old saliva stains and twelve-year-old bone fragments. Good results were obtained even when the template was artificially fragmented into fragments of less than 1 kbp.

引用

页码：23 / 26

页数：4

共 29 条

[1] GENETIC-EVIDENCE EQUATING SRY AND THE TESTIS-DETERMINING FACTOR [J].