PREVALENCE OF COLONIZATION FACTOR ANTIGENS (CFAS) AND ADHERENCE TO HELA-CELLS IN ENTEROTOXIGENIC ESCHERICHIA-COLI ISOLATED FROM FECES OF CHILDREN IN SAO-PAULO

被引：27
作者：
GUTH, BEC
AGUIAR, EG
GRIFFIN, PM
RAMOS, SRTD
GOMES, TAT
机构：
[1] CTR DIS CONTROL,CTR INFECT DIS,DIV BACTERIAL & MYCOT DIS,FOODBORNE & DIARRHEAL DIS BRANCH,ATLANTA,GA 30333
[2] HOSP INFANTIL MENINO JESUS,BR-01329 SAO PAULO,BRAZIL
[3] UNIV SAO PAULO,INST CRIANCA,BR-05403000 SAO PAULO,SP,BRAZIL
来源：
MICROBIOLOGY AND IMMUNOLOGY | 1994年 / 38卷 / 09期
关键词：
ESCHERICHIA COLI; COLONIZATION FACTOR ANTIGENS; ADHERENCE; DIARRHEA;
D O I：
10.1111/j.1348-0421.1994.tb01844.x
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Fifty-eight enterotoxigenic Escherichia coli (ETEC) strains, isolated from children with and without diarrhea in Sao Paulo, were examined for the presence of colonization factor antigens (CFAs) and their ability to adhere to HeLa cells. Antisera to CFA/I, the coli surface (CS) antigens CS1CS3, CS2CS3, and CS2 of CFA/II, CFA/III, and CS5CS6 and CS6 of CFA/IV were used. CFAs were identified in 43% of the ETEC strains: 40% of the strains with CFAs harbored CFA/I, 24% carried CFA/II (CS1CS3), 24%; carried CFA/IV (CS6), and 12% carried CFA/IV (CS5CS6). CFAs occurred mainly among ETEC strains producing only heat-stable (ST-I) enterotoxin and in strains also producing heat-labile toxin (LT-I). No ETEC strains tested expressed CFA/III. A marked change in serotypes of ST-I-producing strains was found in Sao Paulo between 1979 and 1990. Adherence to HeLa cells was detected in 14% of the ETEC strains. All of them had a diffuse adherence pattern and produced only ST-I, and 88% carried CS6 antigen.
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页码：695 / 701
页数：7
相关论文
共 40 条
[1]  
AGUERO ME, 1985, J CLIN MICROBIOL, V22, P576
[2]   COLONIZATION FACTORS OF ENTEROTOXIGENIC ESCHERICHIA-COLI ISOLATED FROM CHILDREN WITH DIARRHEA IN ARGENTINA [J].
BINSZTEIN, N ;
JOUVE, MJ ;
VIBOUD, GI ;
MORAL, LL ;
RIVAS, M ;
ORSKOV, I ;
AHREN, C ;
SVENNERHOLM, AM .
JOURNAL OF CLINICAL MICROBIOLOGY, 1991, 29 (09) :1893-1898
[3]   HEMAGGLUTINATION ACTIVITY AND COLONIZATION FACTOR ANTIGENS-I AND ANTIGEN-II IN ENTERO-TOXIGENIC AND NON-ENTEROTOXIGENIC STRAINS OF ESCHERICHIA-COLI ISOLATED FROM HUMANS [J].
CRAVIOTO, A ;
SCOTLAND, SM ;
ROWE, B .
INFECTION AND IMMUNITY, 1982, 36 (01) :189-197
[4]   ADHESIVE FACTOR FOUND IN STRAINS OF ESCHERICHIA-COLI BELONGING TO THE TRADITIONAL INFANTILE ENTEROPATHOGENIC SEROTYPES [J].
CRAVIOTO, A ;
GROSS, RJ ;
SCOTLAND, SM ;
ROWE, B .
CURRENT MICROBIOLOGY, 1979, 3 (02) :95-99
[5]   ADHESION OF ENTEROTOXIGENIC ESCHERICHIA-COLI TO THE HUMAN COLON-CARCINOMA CELL-LINE CACO-2 IN CULTURE [J].
DARFEUILLE-MICHAUD, A ;
AUBEL, D ;
CHAUVIERE, G ;
RICH, C ;
BOURGES, M ;
SERVIN, A ;
JOLY, B .
INFECTION AND IMMUNITY, 1990, 58 (04) :893-902
[6]   TEST FOR ESCHERICHIA-COLI ENTEROTOXIN USING INFANT MICE - APPLICATION IN A STUDY OF DIARRHEA IN CHILDREN IN HONOLULU [J].
DEAN, AG ;
WILLIAMS, RG ;
CHING, Y ;
HARDEN, LB .
JOURNAL OF INFECTIOUS DISEASES, 1972, 125 (04) :407-&
[7]   PLASMID-CONTROLLED COLONIZATION FACTOR ASSOCIATED WITH VIRULENCE IN ESCHERICHIA-COLI ENTEROTOXIGENIC FOR HUMANS [J].
EVANS, DG ;
SILVER, RP ;
EVANS, DJ ;
CHASE, DG ;
GORBACH, SL .
INFECTION AND IMMUNITY, 1975, 12 (03) :656-667
[8]   HEMAGGLUTINATION OF HUMAN GROUP-A ERYTHROCYTES BY ENTEROTOXIGENIC ESCHERICHIA-COLI ISOLATED FROM ADULTS WITH DIARRHEA - CORRELATION WITH COLONIZATION FACTOR [J].
EVANS, DG ;
EVANS, DJ ;
TJOA, W .
INFECTION AND IMMUNITY, 1977, 18 (02) :330-337
[9]   NEW SURFACE-ASSOCIATED HEAT-LABILE COLONIZATION FACTOR ANTIGEN (CFA-II) PRODUCED BY ENTEROTOXIGENIC ESCHERICHIA-COLI OF SEROGROUP-06 AND SEROGROUP-08 [J].
EVANS, DG ;
EVANS, DJ .
INFECTION AND IMMUNITY, 1978, 21 (02) :638-647
[10]  
EVANS DJ, 1983, REV INFECT DIS, V5, pS692
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