PRELIMINARY CRYSTALLOGRAPHIC DATA FOR PNEUMOCYSTIS-CARINII DIHYDROFOLATE-REDUCTASE

被引：5

作者：

STAMMERS, DK ^{[1
]}

DELVES, C ^{[1
]}

BALLANTINE, S ^{[1
]}

JONES, EY ^{[1
]}

STUART, DI ^{[1
]}

ACHARI, A ^{[1
]}

BRYANT, PK ^{[1
]}

CHAMPNESS, JN ^{[1
]}

机构：

[1] UNIV OXFORD,DEPT BIOCHEM,MOLEC BIOPHYS LAB,OXFORD OX1 3QU,ENGLAND

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1993年 / 230卷 / 02期

关键词：

PNEUMOCYSTIS-CARINII; DHFR; AIDS; CRYSTALS; X-RAY DIFFRACTION;

D O I：

10.1006/jmbi.1993.1183

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Dihydrofolate reductase from Pneumocytis carinii has been crystallized in a form suitable for high resolution X-ray diffraction studies. Recombinant enzyme that had been refolded following solubilization in guanidinium hydrochloride was crystallized as both a ternary complex with the cofactor NADPH and the inhibitor trimethoprim as well as a binary complex with NADPH. The two types of complex crystallized isomorphously from polyethylene glycol using sitting-drop vapour diffusion. The crystals were of space group P 21 with unit cell parameters, a = 69·9 Å, b = 43·6 Å, c = 37·6 Å, β = 117·7°, with one molecule per asymmetric unit. The crystals diffracted to 1·8 Å resolution. © 1993 Academic Press Limited.

引用

页码：679 / 680

页数：2

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