PRELIMINARY CRYSTALLOGRAPHIC DATA FOR PNEUMOCYSTIS-CARINII DIHYDROFOLATE-REDUCTASE

被引:5
作者
STAMMERS, DK [1 ]
DELVES, C [1 ]
BALLANTINE, S [1 ]
JONES, EY [1 ]
STUART, DI [1 ]
ACHARI, A [1 ]
BRYANT, PK [1 ]
CHAMPNESS, JN [1 ]
机构
[1] UNIV OXFORD,DEPT BIOCHEM,MOLEC BIOPHYS LAB,OXFORD OX1 3QU,ENGLAND
关键词
PNEUMOCYSTIS-CARINII; DHFR; AIDS; CRYSTALS; X-RAY DIFFRACTION;
D O I
10.1006/jmbi.1993.1183
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Dihydrofolate reductase from Pneumocytis carinii has been crystallized in a form suitable for high resolution X-ray diffraction studies. Recombinant enzyme that had been refolded following solubilization in guanidinium hydrochloride was crystallized as both a ternary complex with the cofactor NADPH and the inhibitor trimethoprim as well as a binary complex with NADPH. The two types of complex crystallized isomorphously from polyethylene glycol using sitting-drop vapour diffusion. The crystals were of space group P 21 with unit cell parameters, a = 69·9 Å, b = 43·6 Å, c = 37·6 Å, β = 117·7°, with one molecule per asymmetric unit. The crystals diffracted to 1·8 Å resolution. © 1993 Academic Press Limited.
引用
收藏
页码:679 / 680
页数:2
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