PRELIMINARY CRYSTALLOGRAPHIC DATA FOR PNEUMOCYSTIS-CARINII DIHYDROFOLATE-REDUCTASE

被引：5

作者：

STAMMERS, DK ^{[1
]}

DELVES, C ^{[1
]}

BALLANTINE, S ^{[1
]}

JONES, EY ^{[1
]}

STUART, DI ^{[1
]}

ACHARI, A ^{[1
]}

BRYANT, PK ^{[1
]}

CHAMPNESS, JN ^{[1
]}

机构：

[1] UNIV OXFORD,DEPT BIOCHEM,MOLEC BIOPHYS LAB,OXFORD OX1 3QU,ENGLAND

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1993年 / 230卷 / 02期

关键词：

PNEUMOCYSTIS-CARINII; DHFR; AIDS; CRYSTALS; X-RAY DIFFRACTION;

D O I：

10.1006/jmbi.1993.1183

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Dihydrofolate reductase from Pneumocytis carinii has been crystallized in a form suitable for high resolution X-ray diffraction studies. Recombinant enzyme that had been refolded following solubilization in guanidinium hydrochloride was crystallized as both a ternary complex with the cofactor NADPH and the inhibitor trimethoprim as well as a binary complex with NADPH. The two types of complex crystallized isomorphously from polyethylene glycol using sitting-drop vapour diffusion. The crystals were of space group P 21 with unit cell parameters, a = 69·9 Å, b = 43·6 Å, c = 37·6 Å, β = 117·7°, with one molecule per asymmetric unit. The crystals diffracted to 1·8 Å resolution. © 1993 Academic Press Limited.

引用

页码：679 / 680

页数：2

共 50 条

[21] CT IN DISSEMINATED EXTRAPULMONARY PNEUMOCYSTIS-CARINII INFECTION [J].

GAA, J .

FORTSCHRITTE AUF DEM GEBIETE DER RONTGENSTRAHLEN UND DER NEUEN BILDGEBENDEN VERFAHREN, 1991, 155 (05) :474-475

[22] ISOLATED CRANIAL GRANULOMA INDUCED BY PNEUMOCYSTIS-CARINII [J].

BEST, PV ;

SHENOLIKAR, A .

HISTOPATHOLOGY, 1993, 22 (04) :391-394

[23] INVITRO INTERACTION OF HUMAN MACROPHAGES WITH PNEUMOCYSTIS-CARINII [J].

FORTE, M ;

RAHELU, M ;

STUBBERFIELD, C ;

TOMKINS, L ;

PITHIE, A ;

KUMARARATNE, D .

INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY, 1991, 72 (05) :589-598

[24] PNEUMOCYSTIS-CARINII PNEUMONIA - CT AND HRCT OBSERVATIONS [J].

BERGIN, CJ ;

WIRTH, RL ;

BERRY, GJ ;

CASTELLINO, RA .

JOURNAL OF COMPUTER ASSISTED TOMOGRAPHY, 1990, 14 (05) :756-759

[25] IMMUNOHISTOCHEMICAL DETECTION OF PNEUMOCYSTIS-CARINII IN TRANSBRONCHIAL LUNG-BIOPSY SPECIMENS - ANTIGEN DIFFERENCE BETWEEN HUMAN AND RAT PNEUMOCYSTIS-CARINII [J].

KOBAYASHI, M ;

MORIKI, T ;

UEMURA, Y ;

TAKEHARA, N ;

KUBONISHI, I ;

TAGUCHI, H ;

MIYOSHI, I .

JAPANESE JOURNAL OF CLINICAL ONCOLOGY, 1992, 22 (06) :387-392

[26] HYPERCALCEMIA IN A PATIENT WITH AIDS AND PNEUMOCYSTIS-CARINII PNEUMONIA [J].

AHMED, B ;

JASPAN, JB .

AMERICAN JOURNAL OF THE MEDICAL SCIENCES, 1993, 306 (05) :313-316

[27] TRANSMISSION OF PNEUMOCYSTIS-CARINII FROM AIDS PATIENTS TO OTHER IMMUNOSUPPRESSED PATIENTS - A CLUSTER OF PNEUMOCYSTIS-CARINII PNEUMONIA IN RENAL-TRANSPLANT RECIPIENTS [J].

CHAVE, JP ;

DAVID, S ;

WAUTERS, JP ;

VANMELLE, G ;

FRANCIOLI, P .

AIDS, 1991, 5 (08) :927-932

[28] EPIDEMIOLOGY OF PNEUMOCYSTIS-CARINII INFECTION - APPLICATION OF MOLECULAR-GENETIC METHODS [J].

HOPKIN, JM ;

WAKEFIELD, AE .

SAUDI MEDICAL JOURNAL, 1994, 15 (01) :14-16

[29] HISTONE PREOPSONISATION INCREASES THE RESPIRATORY BURST RESPONSE OF PHAGOCYTES TO PNEUMOCYSTIS-CARINII [J].

TAYLOR, MB ;

BROWN, IN .

FEMS MICROBIOLOGY LETTERS, 1991, 90 (01) :79-82

[30] IDENTIFICATION OF PNEUMOCYSTIS-CARINII BY QUICK HEMATOXYLIN AND EOSIN SMEAR [J].

OROZCOFLORIAN, R ;

TRILLO, A .

JOURNAL OF HISTOTECHNOLOGY, 1991, 14 (03) :179-180

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