MONOCLONAL-ANTIBODY DETECTION OF PORCINE MEAT

被引：20
|
作者：
MORALES, P
GARCIA, T
GONZALEZ, I
MARTIN, R
SANZ, B
HERNANDEZ, PE
机构：
[1] Departamento de Nutricion y Bromatologia 111, Facultad de Veterinaria, Universidad Complutense, Madrid
来源：
JOURNAL OF FOOD PROTECTION | 1994年 / 57卷 / 02期
关键词：
D O I：
10.4315/0362-028X-57.2.146
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A stable hybridoma cell line (DD9) has been produced secreting a monoclonal antibody specific for porcine muscle proteins. The DD9 monoclonal antibody (mAb) failed to show a significant cross-reactivity when tested against beef, horse, chicken, and soya proteins, as well as bovine caseins, gelatin, and bovine serum albumin. The DD9 mAb was further used in an indirect ELISA format for detection of defined amounts of porcine meat (1-100%) in beef and chicken meat mixtures immobilized on 96-well plates. Immunorecognition of monoclonal antibodies adsorbed to porcine meat was made with rabbit anti-mouse immunoglobulins conjugated to the enzyme horseradish peroxidase.
引用
收藏
页码：146 / 149
页数：4
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