DEOXYRIBONUCLEIC ACID METHYLASE ACTIVITY IN PEA SEEDLINGS

被引：31

作者：

KALOUSEK, F

MORRIS, NR

机构：

[1] Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences

[2] Department of Pharmacology, Rutgers Medical School, New Brunswick

来源：

SCIENCE | 1969年 / 164卷 / 3880期

关键词：

D O I：

10.1126/science.164.3880.721

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Deoxyribonucleic acid methylase activity has been detected in a preparation of disrupted nuclei prepared from pea seedlings. S-Adenosyl-L-methionine acted as a donor of methyl groups, and the product of the reaction was identified as 5-methylcytosine. The reaction had a sharp temperature optimum at about 30°C and was unusual in that the DNA methylase was able to methylate DNA in the crude extract.

引用

页码：721 / &

共 15 条

[1]

Chargaff E, 1955, NUCLEIC ACIDS, P307

[2] OCCURRENCE OF 6-METHYLAMINOPURINE IN DEOXYRIBONUCLEIC ACIDS [J].