SURVIVAL AND VIABILITY OF FRESH AND FROZEN-THAWED IN-VITRO BOVINE BLASTOCYSTS

被引：49

作者：

MASSIP, A ^{[1
]}

MERMILLOD, P ^{[1
]}

VANLANGENDONCKT, A ^{[1
]}

TOUZE, JL ^{[1
]}

DESSY, F ^{[1
]}

机构：

[1] INRA, CTR RECH TOURS, F-37380 NOUZILLY, FRANCE

来源：

REPRODUCTION NUTRITION DEVELOPMENT | 1995年 / 35卷 / 01期

关键词：

BOVINE; IN VITRO; EMBRYO; FREEZING; TRANSFER;

D O I：

10.1051/rnd:19950101

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Three experiments were undertaken to investigate the effect of some factors on survival and viability of bovine blastocysts produced in vitro in serum-free oviduct-conditioned media. In vitro survival to freezing was higher for blastocysts appearing on days 6 and 7 than for day 8 blastocysts (67 and 50% hatching rate vs 12%). Transfer success of day 8 blastocysts to recipient heifers was 35% (9/26) carves born vs 19% (4/21) for cows. Embryo mortality occurred mainly during the periimplantation period (between days 21 and 45) for cows compared with heifers. Calving rate-after direct transfer of frozen blastocysts without dilution was 24% (4/17) compared with 31% (5/16) for control unfrozen blastocysts.

引用

页码：3 / 10

页数：8

共 37 条

[1] MORPHOLOGICAL DEVELOPMENT AND SEX OF BOVINE INVITRO-FERTILIZED EMBRYOS [J].