FLUORESCENCE INSITU HYBRIDIZATION MAPPING OF HUMAN CHROMOSOME-19 - CYTOGENETIC BAND LOCATION OF 540 COSMIDS AND 70 GENES OR DNA MARKERS

被引：100

作者：

TRASK, B

FERTITTA, A

CHRISTENSEN, M

YOUNGBLOM, J

BERGMANN, A

COPELAND, A

DEJONG, P

MOHRENWEISER, H

OLSEN, A

CARRANO, A

TYNAN, K

机构：

[1] LAWRENCE LIVERMORE NATL LAB,DIV BIOMED SCI,CTR HUMAN GENOME,LIVERMORE,CA 94550

[2] CALIF STATE UNIV STANISLAUS,DEPT BIOL,TURLOCK,CA 95380

来源：

GENOMICS | 1993年 / 15卷 / 01期

关键词：

D O I：

10.1006/geno.1993.1021

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We report here the band location of 540 cosmids mapped to chromosome 19. The cosmids were mapped by fluorescence in situ hybridization (FISH) relative to chromosomal bands produced by DAPI/actinomycin staining. The cosmids are distributed throughout the chromosome, with a sampling bias for the q-arm. A detailed analysis of the distribution of three different subtelomeric and 22 pericentromeric chromosome 19 cosmids on other chromosomes is also reported. Colony hybridization identified 142 cosmids that contain sequences representing genes or DNA markers that map to chromosome 19. FISH mapping of these cosmids sublocalizes a total of 70 genes and DNA markers on chromosome 19, revises the previously published map assignments of 2 genes, and narrows the location of over 20 markers. © 1993 Academic Press, Inc.

引用

页码：133 / 145

页数：13

共 83 条

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