A RECOMBINANT VECTOR DERIVED FROM THE HOST RANGE-RESTRICTED AND HIGHLY ATTENUATED MVA STRAIN OF VACCINIA VIRUS STIMULATES PROTECTIVE IMMUNITY IN MICE TO INFLUENZA-VIRUS

被引：244

作者：

SUTTER, G ^{[1
]}

WYATT, LS ^{[1
]}

FOLEY, PL ^{[1
]}

BENNINK, JR ^{[1
]}

MOSS, B ^{[1
]}

机构：

[1] NIAID,VIRAL DIS LAB,BETHESDA,MD 20892

来源：

VACCINE | 1994年 / 12卷 / 11期

关键词：

INFLUENZA VIRUS; VACCINIA VIRUS; MVA STRAIN; HOST-RANGE RESTRICTION;

D O I：

10.1016/0264-410X(94)90341-7

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The immunogenicity of a recombinant vir us derived from modified vaccinia virus Ankara (MVA), a host range-restricted highly attenuated and safety-tested strain, was investigated. Plasmid transfer vectors that provide strong synthetic early/late promoters for the simultaneous expression of two genes as well as a transient or stable selectable marker and flanking sequences for homologous recombination with the MVA genome were constructed. A recombinant MVA containing influenza virus haemagglutinin and nucleoprotein genes was isolated in avian cells and shown to express both proteins efficiently upon infection of human or mouse cells in which abortive replication occurs. Mice, inoculated by various routes with recombinant MVA, produced antibody and cytotoxic T-lymphocyte responses to influenza virus proteins and were protected against a lethal influenza virus challenge as effectively as mice immunized with a recombinant derived from the replication-competent WR strain of vaccinia virus.

引用

页码：1032 / 1040

页数：9

共 28 条

[1] THE ROLES OF INFLUENZA-VIRUS HEMAGGLUTININ AND NUCLEOPROTEIN IN PROTECTION - ANALYSIS USING VACCINIA VIRUS RECOMBINANTS [J].