CRYSTALLIZATION AND PRELIMINARY-X-RAY DIFFRACTION STUDIES OF BACILLUS-STEAROTHERMOPHILUS FARNESYL DIPHOSPHATE SYNTHASE EXPRESSED IN ESCHERICHIA-COLI

被引：11

作者：

NAKANE, H

KOYAMA, T

OBATA, S

OSABE, M

TAKESHITA, A

NISHINO, T

OGURA, K

MIKI, K

机构：

[1] TOKYO INST TECHNOL,RESOURCES UTILIZAT RES LAB,MIDORI KU,YOKOHAMA,KANAGAWA 227,JAPAN

[2] TOYOTA MOTOR CO LTD,BIORES LAB,TOYOTA,AICHI 471,JAPAN

[3] TOHOKU UNIV,INST CHEM REACT SCI,AOBA KU,SENDAI,MIYAGI 980,JAPAN

[4] TOHOKU UNIV,FAC ENGN,DEPT BIOCHEM & ENGN,AOBA KU,SENDAI,MIYAGI 980,JAPAN

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1993年 / 233卷 / 04期

关键词：

FARNESYL DIPHOSPHATE SYNTHASE; THERMOSTABLE ENZYME; PRENYLTRANSFERASE; CRYSTALLIZATION; X-RAY DIFFRACTION;

D O I：

10.1006/jmbi.1993.1554

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Thermostable farnesyl diphosphate synthase (EC 2.5.1.10) from Bacillus stearothermophilus, which was overexpressed in Escherichia coli, has been crystallized by the vapor-diffusion procedure. Tetragonal crystals were obtained using ammonium sulfate as a precipitant. The crystals diffracted X-rays to about 3 Å resolution. The diffraction pattern indicated that the space group is I4122 with unit-cell dimensions of a = b = 114 Å and c = 247 Å. It is thought that the asymmetric unit comprises two or three molecules of farnesyl diphosphate synthase. © 1993 Academic Press Limited.

引用

页码：787 / 788

页数：2

共 50 条

[1] CRYSTALLIZATION AND PRELIMINARY-X-RAY DIFFRACTION STUDIES OF THE COBALAMIN-BINDING DOMAIN OF METHIONINE SYNTHASE FROM ESCHERICHIA-COLI
LUSCHINSKY, CL
DRUMMOND, JT
MATTHEWS, RG
LUDWIG, ML
JOURNAL OF MOLECULAR BIOLOGY, 1992, 225 (02) : 557 - 560
[2] CRYSTALLIZATION AND PRELIMINARY-X-RAY DIFFRACTION STUDIES ON THE APO FORM OF OROTATE PHOSPHORIBOSYLTRANSFERASE FROM ESCHERICHIA-COLI
AGHAJARI, N
JENSEN, KF
GAJHEDE, M
JOURNAL OF MOLECULAR BIOLOGY, 1994, 241 (02) : 292 - 294
[3] CRYSTALLIZATION AND PRELIMINARY-X-RAY CRYSTALLOGRAPHIC ANALYSIS OF THE SOYBEAN PROGLYCININ EXPRESSED IN ESCHERICHIA-COLI
UTSUMI, S
GIDAMIS, AB
MIKAMI, B
KITO, M
JOURNAL OF MOLECULAR BIOLOGY, 1993, 233 (01) : 177 - 178
[4] CRYSTALLIZATION AND PRELIMINARY-X-RAY INVESTIGATION OF ESCHERICHIA-COLI PORPHOBILINOGEN DEAMINASE
JORDAN, PM
WARREN, MJ
MGBEJE, BIA
WOOD, SP
COOPER, JB
LOUIE, G
BROWNLIE, P
LAMBERT, R
BLUNDELL, TL
JOURNAL OF MOLECULAR BIOLOGY, 1992, 224 (01) : 269 - 271
[5] CRYSTALLIZATION AND PRELIMINARY-X-RAY DIFFRACTION STUDIES OF THE ENOYL-ACP REDUCTASE FROM ESCHERICHIA-COLI
WAGNER, UG
BERGLER, H
FUCHSBICHLER, S
TURNOWSKY, F
HOGENAUER, G
KRATKY, C
JOURNAL OF MOLECULAR BIOLOGY, 1994, 243 (01) : 126 - 127
[6] CRYSTALLIZATION AND PRELIMINARY-X-RAY STUDIES OF L-ASPARTASE FROM ESCHERICHIA-COLI
SHI, WX
KIDD, R
GIORGIANNI, F
SCHINDLER, JF
VIOLA, RE
FARBER, GK
JOURNAL OF MOLECULAR BIOLOGY, 1993, 234 (04) : 1248 - 1249
[7] CRYSTALLIZATION AND PRELIMINARY-X-RAY DIFFRACTION STUDY OF THE NICKEL-BINDING PROTEIN NIKA OF ESCHERICHIA-COLI
CHARON, MH
WU, LF
PIRAS, C
DEPINA, K
MANDRANDBERTHELOT, MA
FONTECILLACAMPS, JC
JOURNAL OF MOLECULAR BIOLOGY, 1994, 243 (02) : 353 - 355
[8] CRYSTALLIZATION AND PRELIMINARY-X-RAY INVESTIGATION OF HOLOTRYPTOPHANASES FROM ESCHERICHIA-COLI AND PROTEUS-VULGARIS
DEMENTIEVA, IS
ZAKOMIRDINA, LN
SINITZINA, NI
ANTSON, AA
WILSON, KS
ISUPOV, MN
LEBEDEV, AA
HARUTYUNYAN, EH
JOURNAL OF MOLECULAR BIOLOGY, 1994, 235 (02) : 783 - 786
[9] CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF THE 2 DOMAINS OF GLUCOSAMINE-6-PHOSPHATE SYNTHASE FROM ESCHERICHIA-COLI
OBMOLOVA, G
BADETDENISOT, MA
BADET, B
TEPLYAKOV, A
JOURNAL OF MOLECULAR BIOLOGY, 1994, 242 (05) : 703 - 705
[10] CRYSTALLIZATION AND PRELIMINARY-X-RAY DIFFRACTION STUDIES OF HUMAN RANTES
SHAW, JP
KRYGER, G
CLEASBY, A
WONACOTT, A
POWER, CA
PROUDFOOT, AEI
WELLS, TNC
JOURNAL OF MOLECULAR BIOLOGY, 1994, 242 (04) : 589 - 590

← 1 2 3 4 5 →