AN ASSAY FOR GLUCOSE 6-PHOSPHATASE BASED ON THE FORMATION OF GLUCOSE

被引：0
|
作者：
BUBLITZ, C
机构：
来源：
MOLECULAR AND CELLULAR BIOCHEMISTRY | 1991年 / 108卷 / 02期
关键词：
GLUCOSE; 6-PHOSPHATASE; 6-PHOSPHATE; DOWEX-2; FLUORIDE;
D O I：
暂无
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
A rapid, convenient method for the assay of glucose 6-phosphatase dependent on the removal of radioactive substrate from radioactive product by Dowex 2 fluoride is described. The enzymatic reaction is stopped by the addition of an ethanolic slurry of the resin. After the tubes are shaken, the radioactivity of glucose in the clarified supernatant layer is measured. The release of glucose is directly proportional to time and enzyme concentration. Detergents do not interfere with the method.
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页码：141 / 144
页数：4
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