CALLUS FORMATION FROM ISOLATED GLOBE ARTICHOKE (CYNARA-SCOLYMUS L) SUSPENSION PROTOPLASTS

被引：11

作者：

ORDAS, RJ

TAVAZZA, R

ANCORA, G

机构：

[1] EUROPEAN NACL ENERGIA ATOM,DIPARTIMENTO AGROBIOTECHNOL,DIV INGN GENET,CP 2400,ROME,ITALY

[2] UNIV OVIEDO,FAC BIOL,FISIOL VEGETAL LAB,OVIEDO,SPAIN

来源：

PLANT SCIENCE | 1991年 / 77卷 / 02期

关键词：

CYNARA-SCOLYMUS L; PROTOPLAST; SUSPENSION CULTURE; GLOBE ARTICHOKE;

D O I：

10.1016/0168-9452(91)90094-O

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Viable protoplasts were isolated from suspension culture cells of globe artichoke (Cynara scolymus L.). Protoplast yield, cell wall regeneration and cell division were influenced by several factors, e.g. age of the cell culture, enzyme composition, culture density. First cell divisions were observed after 4-6 days of culture. Upon transfer to solid medium, the cell colonies gave rise to proliferating green calli. Globular structures formed on these calli but failed to develop further.

引用

页码：253 / 259

页数：7

共 9 条

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ALATABEE JS, 1990, ACTA HORTIC, V280, P255

[2]

Ancora G., 1986, Biotechnology in agriculture and forestry 2. Crops I, P471

[3]

DIXON RA, 1985, PLANT CELL CULTURE P

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